▼ SeqCP parameters for initial filtration

Sequence length: ≥ 100 (Default) < 100

Length ratio: ≥ 0.9 ≥ 0.8 (Default) ≥ 0.7

RSB (Relative Shift of aligned Boundaries): ≥ 0.1 ≥ 0.2 (Default) ≥ 0.3 ≥ 0.4

IDA(Identity Difference between Alignments): ≤ 0.1 (Default) ≤ 0.2 ≤ 0.3

Maximal proportion of flanks: < 0.1 < 0.2 (Default) < 0.3 < 0.4

Alignment boundary bias: ≤ 0.1 ≤ 0.2 (Default) ≤ 0.3 ≤ 0.4

Alignment rate of the duplicated query alignment: ≥ 0.9 ≥ 0.8 (Default) ≥ 0.7 ≥ 0.6

Permutation size: ≥ 0.1 ≥ 0.2 (Default) ≥ 0.3

GR_cp (Alignment gap for CP identification): < 20 < 30 (Default) < 40

Chen CC, Huang YW, Huang HC, Lo WC, Lyu CP. (2023) SeqCP: A sequence-based algorithm for searching circularly permuted proteins.Comput Struct Biotechnol J. 21:185-201.